Genetic toolbox for controlled expression of functional proteins in Geobacillus spp.
نویسندگان
چکیده
منابع مشابه
Genetic toolbox for controlled expression of functional proteins in Geobacillus spp.
Species of genus Geobacillus are thermophilic bacteria and play an ever increasing role as hosts for biotechnological applications both in academia and industry. Here we screened a number of Geobacillus strains to determine which industrially relevant carbon sources they can utilize. One of the strains, G. thermoglucosidasius C56-YS93, was then chosen to develop a toolbox for controlled gene ex...
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D-amino acid aminotransferases (D-AATs) from Geobacillus toebii SK1 and Geobacillus sp. strain KLS1 were cloned and characterized from a genetic, catalytic, and structural aspect. Although the enzymes were highly thermostable, their catalytic capability was approximately one-third of that of highly active Bacilli enzymes, with respective turnover rates of 47 and 55 s(-1) at 50 degrees C. The Ge...
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1. School of Computer Science and Technology, Xidian University, Xi’an710071, Shannxi, PR China 2. Institute of Science and Technology for Brain Inspired Intelligence, Fudan University, Shanghai, PR China 3. Department of Computer Science, University of Warwick, Coventry CV4 7AL, UK 4. Oxford Centre for Computational Neuroscience, Oxford UK 5. Center for Magnetic Resonance Research (CMRR), Univ...
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Libraries of well-characterised components regulating gene expression levels are essential to many synthetic biology applications. While widely available for the Gram-negative model bacterium Escherichia coli, such libraries are lacking for the Gram-positive model Bacillus subtilis, a key organism for basic research and biotechnological applications. Here, we engineered a genetic toolbox compri...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2017
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0171313